此文为优先出版论文,建议参考文献书写格式:杜彩丽, 张玮, 张军毅, 尚光霞, 杨丽, 王梦梦, 王丽卿, . 蟛蜞菊水提液对水华蓝藻——卵孢金孢藻的化感作用研究[J/OL]. 生态毒理学报, 2019 (02). http://www.cnki.net/kcms/detail/11.5470.X.20181113.1319.002.html

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蟛蜞菊水提液对水华蓝藻——卵孢金孢藻的化感作用研究

Allelopathic Effects of Wedelia chinensis Water Extracts on the Cyanobacterium Chrysosporum ovalisporum,a Species Causing Algal Bloom

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【作者】 杜彩丽张玮张军毅尚光霞杨丽王梦梦王丽卿

【Author】 Du Caili;Zhang Wei;Zhang Junyi;Shang Guangxia;Yang Li;Wang Mengmeng;Wang Liqing;Centre for Research on Environmental Ecology and Fish Nutrient (CREEFN) of the Ministry of Agriculture,Shanghai Ocean University;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University;Wuxi Environmental Monitoring Centre Station;

【通讯作者】 张玮;王丽卿;

【机构】 上海海洋大学农业部鱼类营养与环境生态研究中心上海海洋大学水产种质资源发掘与利用教育部重点实验室上海海洋大学水产科学国家级实验教学示范中心无锡市环境监测中心站

【摘要】 为探究陆生植物对水华蓝藻的化感作用,本文采用不同浓度的蟛蜞菊(Wedelia chinensis)水提液对卵孢金孢藻(Chrysosporum ovalisporum)进行了为期6 d的室内培养试验,结果发现:蟛蜞菊水提液对卵孢金孢藻的化感作用总体上呈"低促高抑";当蟛蜞菊水提液浓度大于6 g·L-1时,将显著降低卵孢金孢藻光合作用有效量子效率(YⅡ)、最大量子产量(Fv/Fm)、叶绿素a(Chla)含量和藻细胞生物量(P<0.01);当浓度大于8 g·L-1时,单位藻细胞内的超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)活性显著高于对照组(P<0.05),表明蓝藻细胞受到较强的胁迫作用;透射电镜观察结果表明:高浓度蟛蜞菊水提液会破坏细胞膜,导致藻细胞内的类囊体、羧基体、气囊等超微结构受损,并最终导致藻细胞溶解、藻丝断裂和死亡。此外,本文利用Logisitic模型研究了蟛蜞菊水提液对卵孢金孢藻的半效应浓度(EC50),其24 h、48 h、72 h和144 h的EC50分别为:3.78 g·L-1、4.47 g·L-1、4.72 g·L-1和6.55 g·L-1。本研究为中国湖泊蓝藻水华的防治增添了基础数据和新的技术思路。

【Abstract】 In order to explore the allelopathic effects of terrestrial plants on cyanobacterial species that cause algal bloom, Chrysosporum ovalisporum was exposed to different concentrations of Wedelia chinensis water extracts during a 6-day incubation. The results showed that low concentration extracts of W. chinensis significantly improved the growth of C. ovalisporum, while the high concentrations extracts showed significantly negative effect on C. ovalisporum. The values of potential maximal photochemical efficiency of PSⅡ(Fv/Fm), effective photochemical quantum yield of PSⅡ(YⅡ) and chlorophyll-a(Chl-a) were significantly reduced by the extracts when its concentrations were greater than 6 g·L-1(P<0.01). Compared with the control group, the superoxide dismutase(SOD) and gutathione-S-transferase(GST) activity of C. ovalisporum in high concentration W. chinensis extracts treatment group(8 g·L-1) increased significantly(P<0.05), which indicated that C. ovalisporum survive extreme environmental stress.Transmission electron microscope(TEM) pictures showed that ultra-structures of C. ovalisporum cells, such as thylakoid, carboxyl, gas vesicles and cell membranes were destroyed by the extracts of W. chinensis, consequently, resulting in the dissolution, fracture of C. ovalisporum filaments. In addition, the median effect concentration(EC50) of W. chinensis water extracts in 24 h, 48 h, 72 h and 144 h were 3.78 g·L-1, 4.47 g·L-1, 4.72 g·L-1 and 6.55 g·L-1,respectively, estimated by using Logisitic growth model. Our study contributes to the existing knowledge and data of freshwater cyanobacterial bloom, and provides insights for its control and freshwater system restoration infected by cyanobacterial bloom.

【基金】 上海市优秀技术带头人计划项目(15XD1522900);上海水务局河道生态监测项目(沪水科2012-2)
  • 【文献出处】 生态毒理学报 ,Asian Journal of Ecotoxicology , 编辑部邮箱 ,2019年02期
  • 【分类号】X524
  • 【网络出版时间】2018-11-13 13:19
  • 【下载频次】161
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