此文为优先出版论文,建议参考文献书写格式:张明川, 张玲, 马一鸣, 程淑霞, 王莉, . miR-199a-5p下调NF-κB信号通路激活水平抑制宫颈癌细胞恶性生物学行为[J/OL]. 中国医药生物技术, 2019 (04). http://www.cnki.net/kcms/detail/11.5512.R.20190529.0921.002.html

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miR-199a-5p下调NF-κB信号通路激活水平抑制宫颈癌细胞恶性生物学行为

miR-199a-5p down-regulates the activation level of NF-κB signaling pathway and inhibits malignant biological behavior of cervical cancer cells

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【作者】 张明川张玲马一鸣程淑霞王莉

【Author】 ZHANG Ming-chuan;ZHANG Ling;MA Yi-ming;CHENG Shu-xia;WANG Li;Department of Gynecologic Oncology,Cancer Hospital Affiliated to Zhengzhou University,Henan Cancer Hospital;Department of Surgical Oncology,The Second People’s Hospital of Hami;

【通讯作者】 王莉;

【机构】 郑州大学附属肿瘤医院妇瘤科哈密市第二人民医院肿瘤外科

【摘要】 目的探讨miR-199a-5p对宫颈癌细胞生长、侵袭和迁移的影响和机制。方法 Real-time PCR测定miR-199a-5p在宫颈癌HeLa、HCC94、Ca Ski细胞和正常宫颈Ect1/E6E7细胞中的表达差异。在宫颈癌细胞中转染miR-199a-5p mimics,real-time PCR检测过表达效果,MTT检测增殖,克隆形成实验测定克隆形成能力,Transwell小室测定侵袭和迁移能力,Westernblot检测细胞中p-p65、p-IκB蛋白表达变化。使用核因子-κB(NF-κB)激活剂PMA处理转染miR-199a-5p mimics后的宫颈癌细胞,同样使用上述方法测定细胞生长、克隆、侵袭和迁移能力变化。结果 miR-199a-5p在宫颈癌HeLa、HCC94、CaSki细胞中的表达水平低于正常宫颈Ect1/E6E7细胞。miR-199a-5p mimics提高宫颈癌细胞中miR-199a-5p表达水平,降低细胞增殖、克隆、侵袭和迁移能力,减少细胞中p-p65、p-IκB蛋白表达。NF-κB激活剂PMA可以逆转miR-199a-5p对宫颈癌细胞增殖、克隆、侵袭和迁移能力的抑制作用。结论 miR-199a-5p通过下调NF-κB信号通路激活水平抑制宫颈癌细胞生长、侵袭和迁移。

【Abstract】 Objective To investigate the effects and mechanisms of miR-199 a-5 p on the growth, invasion and migration of cervical cancer cells.Methods Real-time PCR was used to detect the expression of miR-199 a-5 p in He La, HCC94, CaSki cells and normal cervical Ect1/E6 E7 cells. miR-199 a-5 p mimics were transfected into cervical cancer cells. Real-time PCR was used to detect the overexpression effect. MTT assay was used to detect proliferation and the colony formation ability was determined by clone formation test. Transwell chamber was used to measure invasion and migration ability. Western blot was used to detect the expression of p-p65 and p-IκB protein. The cervical cancer cells transfected with miR-199 a-5 p mimics were treated with PMA, an activator of NF-κB. The changes of cell growth, colony formation, invasion and migration ability were also measured by the above methods.Results The expression level of miR-199 a-5 p in HeLa, HCC94 and CaSki cells was lower than that in normal cervical Ect1/E6 E7 cells. miR-199 a-5 p mimics increased the expression of miR-199 a-5 p in cervical cancer cells and reduced cell proliferation, colony formation, invasion and migration, and the expression of p-p65 and p-IκB protein in cells. PMA, an activator of NF-κB, could reverse the inhibitory effect of miR-199 a-5 p on proliferation, colony formation, invasion and migration of cervical cancer cells.Conclusion miR-199 a-5 p down-regulates the activation level of NF-kB signaling pathway and inhibits the growth, invasion and migration of cervical cancer cells.

  • 【文献出处】 中国医药生物技术 ,Chinese Medicinal Biotechnology , 编辑部邮箱 ,2019年04期
  • 【分类号】R737.33
  • 【网络出版时间】2019-05-29 09:21
  • 【下载频次】88
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