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L-色氨酸发酵中的细胞循环工艺研究

Development of a cell cycle technique in L-tryptophan fermentation

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【作者】 李静刘晓东滕佳佳

【Author】 LI Jing;LIU Xiaodong;TENG Jiajia;Henan Julong Biological Engineering Co., Ltd.;

【机构】 河南巨龙生物工程股份有限公司

【摘要】 在L-色氨酸发酵过程中,为了提高生物量及细胞的利用率、目的产物产量及糖酸转化率,研究了发酵罐与碟片式分离机相偶联的细胞循环发酵模式,通过分析不同的细胞循环策略及其对生物量、L-色氨酸产量与糖酸转化率的影响,确定了最佳的细胞循环培养策略。结果表明:当采用V(上清液)∶V(浓缩液)=1∶1、细胞循环周期为20~26 h的循环培养策略时,发酵生物量(55.26 g/L)、L-色氨酸产量(52.45 g/L),糖酸转化率(19.75%)均最高;副产物乙酸积累量(0.87 g/L)、谷氨酸生成量(4.24 g/L)及NH+4浓度(86.52 mmol/L)均最低。因此,细胞循环发酵可提高生物量、L-色氨酸产量及糖酸转化率,降低副产物积累量及缩短发酵周期。

【Abstract】 In order to improve the seed biomass and cell utilization of L-tryptophan fermentation process, and further increase the target product yield and sugar acid conversion, we developed a cell cycle fermentation mode coupled with a fermentation tank and a disc separator. Through the investigation of different cell cycle strategies and their effects on biomass, L-tryptophan yield and sugar acid conversion, the optimal cell cycle culture strategy was determined. When the cycle culture strategy of V(supernatant)∶V(concentrate)=1∶1 and the cell cycle of 20-26 h was adopted, the fermentation biomass(55.26 g/L), L-tryptophan yield(52.45 g/L), and the highest sugar-acid conversion(19.75%) were achieved. Meanwhile, the lowest acetic acid accumulation(0.87 g/L), glutamic acid production(4.24 g/L) and NH+4 concentration(86.52 mmol/L) were reached. Therefore, the developed cell cycle fermentation technique improved biomass, L-tryptophan yield and sugar-acid conversion, and reduced by-product accumulation and shorten fermentation cycle.

【关键词】 大肠杆菌L-色氨酸发酵细胞循环
【Key words】 Escherichia coliL-tryptophanfermentationcell recycle
  • 【文献出处】 发酵科技通讯 ,Bulletin of Fermentation Science and Technology , 编辑部邮箱 ,2021年03期
  • 【分类号】TQ922
  • 【下载频次】136
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