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邻苯二甲酸二丁酯对小鼠腹腔巨噬细胞的影响

Effect of di-n-butyl phthalate on peritoneal macrophages in mice

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【作者】 向先兰张也刘蕾杨湘梅魏家兴武阳晏彪马萍

【Author】 XIANG Xian-lan;ZHANG Ye;LIU Lei;YANG Xiang-mei;WEI Jia-xing;WU Yang;YAN Biao;MA Ping;Lab of Environment-Immunological and Neurological Diseases, School of Basic Medical Sciences,Hubei University of Science and Technology;

【通讯作者】 马萍;

【机构】 湖北科技学院基础医学院环境-免疫与神经系统疾病实验室

【摘要】 目的研究邻苯二甲酸二丁酯(dibutyl phthalate,DBP)对小鼠腹腔巨噬细胞的影响。方法昆明雌性小鼠腹腔巨噬细胞,经6种不同浓度(0、6.25、12.5、25、50、100μmol/L)的DBP分别暴露12、24 h后,观察细胞形态学变化,测定细胞活性(CCK-8)、乳酸脱氢酶(LDH)、活性氧(ROS)及丙二醛(MDA)水平,观察50μmol/L维生素E(vitamin E,VE)对100μmol/L DBP所致氧化损伤的拮抗。结果随着DBP暴露剂量的增加,较高剂量的DBP(≥25μmol/L)可使小鼠巨噬细胞产生过量的ROS(P<0.01),MDA含量明显上升(P<0.01),同时伴有小鼠巨噬细胞的CCK-8含量下降(P<0.01)及LDH释放增加(P<0.01);与100μmol/L DBP组比较,100μmol/L DBP+50μmol/L VE组CCK-8含量上升,LDH、ROS及MDA水平均明显下降(P<0.01)。结论结果表明,较高剂量(≥25μmol/L)的DBP可引起小鼠腹腔巨噬细胞的氧化损伤,致巨噬细胞活性降低和细胞膜破坏;维生素E作为抗氧化剂,在一定程度上可拮抗DBP造成的巨噬细胞损伤。

【Abstract】 Objective To explore the effect of dibutyl phthalate(DBP) on peritoneal macrophages in mice. Methods The peritoneal macrophages of Kunming female mice were exposed to DBP with six different concentrations of 0, 6.25, 12.5, 25, 50 and 100 μmol/L for 12 h and 24 h, respectively.The morphological changes of mouse peritoneal macrophages were observed.Meanwhile the cell activity(CCK-8), the activities of lactate dehydrogenase(LDH), the levels of reactive oxygen species(ROS)and malondialdehyde(MDA) were also measured among mouse peritoneal macrophages exposed to DBP. The antioxidant effects of 50 μmol/L vitamin E(VE) on oxidative damage of mouse peritoneal macrophages induced by 100 μmol/L DBP were observed. Results With the increase of exposure dose of DBP, higher doses(≥25 μmol/L) of DBP induced excessive ROS(P<0.01) and increasing MDA content(P <0.01) in mouse peritoneal macrophages, accompanied with the decrease of CCK-8 content(P<0.01) and the increase of LDH release(P<0.01).Compared with the 100 μmol/L DBP group, the content of CCK-8 increased, and the levels of LDH, ROS and MDA decreased significantly in 100 μmol/L DBP+50 μmol/L VE group(P<0.01).Conclusion Higher doses(≥25 μmol/L) of DBP can cause oxidative damage of mouse peritoneal macrophages, decrease of macrophage activity and destruction of cell membrane. However, vitamin E as an antioxidant, can antagonize the macrophage damage induced by DBP to some extent.

【基金】 湖北省大学生创新训练计划项目(201710927028);湖北省卫生计生委重点支撑项目(WJ2017Z027);湖北省高等学校优秀中青年科技创新团队计划项目(T201717)
  • 【文献出处】 环境与健康杂志 ,Journal of Environment and Health , 编辑部邮箱 ,2018年10期
  • 【分类号】R114
  • 【下载频次】29
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