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3种黄酮类化合物对铝染毒大鼠肝肾抗氧化系统的保护作用

Protective effects of three kinds of flavonoids on antioxidant system in liver and kidney tissues of rats exposed to aluminum

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【作者】 董艳张雪娇陈梦迪任娜聂爽刘萍

【Author】 DONG Yan;ZHANG Xue-jiao;CHEN Meng-di;REN Na;NIE Shuang;LIU Ping;Department of Nuclear Medicine,Qilu Hospital of Shandong University;

【通讯作者】 刘萍;

【机构】 山东大学齐鲁医院核医学科山东大学公共卫生学院理化检验学系

【摘要】 目的探讨黄酮类化合物对铝染毒大鼠肝肾抗氧化系统的保护作用。方法将64只健康SPF级雄性Wistar大鼠按体重随机分为8组,分别为阴性对照组、三氯化铝染毒组和100、200 mg/kg芦丁、葛根素、水飞蓟素拮抗组,每组8只。采用灌胃方式进行染毒,每天1次,其中,阴性对照组连续12周给予1.0 ml/d生理盐水;前4周三氯化铝染毒组和各剂量芦丁、葛根素、水飞蓟素拮抗组分别给予281.40 mg/kg三氯化铝溶液;后8周分别给予1 ml生理盐水及相应剂量的黄酮类化合物。测定大鼠肝、肾组织中的丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、ATP酶(ATPase)活力。结果与阴性对照组相比,三氯化铝染毒组大鼠肝、肾组织中的MDA含量升高,而SOD、GSH-Px活力及Na+K+-ATP酶、Mg2+-ATP酶、Ca2+-ATP酶活力均下降,差异均有统计学意义(P<0.05)。与三氯化铝染毒组比较,200 mg/kg芦丁拮抗组大鼠肝组织中的SOD活力以及200 mg/kg芦丁、葛根素拮抗组和各剂量水飞蓟素拮抗组大鼠肝组织中的GSH-Px活力均升高,差异均有统计学意义(P<0.05);而各剂量芦丁、葛根素、水飞蓟素拮抗组大鼠肝组织中的MDA含量均无明显变化。与三氯化铝染毒组比较,各剂量芦丁、葛根素、水飞蓟素拮抗组大鼠肾组织中的MDA含量均较低,而200mg/kg芦丁拮抗组大鼠肾组织中的SOD、GSH-Px活力升高,差异均有统计学意义(P<0.05)。与三氯化铝染毒组比较,200mg/kg芦丁、水飞蓟素拮抗组大鼠肝组织中的Na+K+-ATP酶活力及200 mg/kg芦丁、葛根素、水飞蓟素拮抗组大鼠肝组织中的Ca2+-ATP酶活力以及200 mg/kg葛根素、水飞蓟素拮抗组和各剂量芦丁拮抗组大鼠肝组织中的Mg2+-ATP酶活力均较高,差异有统计学意义(P<0.05)。与三氯化铝染毒组比较,200 mg/kg芦丁、水飞蓟素拮抗组大鼠肾组织中的Na+K+-ATP酶活力及200 mg/kg葛根素、水飞蓟素拮抗组大鼠肾组织中的Ca2+-ATP酶活力以及200 mg/kg芦丁拮抗组和各剂量水飞蓟素拮抗组大鼠肾组织中的Mg2+-ATP酶活力均较高,差异有统计学意义(P<0.05)。且随着芦丁、葛根素、水飞蓟素处理剂量的升高,三氯化铝染毒大鼠肝、肾组织中的MDA含量均呈下降趋势,而SOD、GSH-Px活力和Na+K+-ATP酶、Mg2+-ATP酶、Ca2+-ATP酶活力均呈上升趋势。结论黄酮类化合物(芦丁、葛根素、水飞蓟素)在一定剂量范围内对铝染毒大鼠肝肾组织抗氧化系统具有保护作用,可以有效地减轻铝造成的肝肾损伤。

【Abstract】 Objective To investigate the protective effects of flavonoids on the antioxidation system in liver and kidney tissues of rats exposed to aluminum.Methods Sixty-four male Wistar rats were randomly divided into eight groups,eight in each,including control group,aluminum group,100 mg/kg rutin group,200 mg/kg rutin group,100 mg/kg puerarin group,200 mg/kg puerarin group,100 mg/kg silymarin group,200 mg/kg silymarin group.The tested chemicals were given through gavage once a day.The rats in control group received 1.0 ml/d saline for twelve weeks.Aluminum group and other flavonoids intervention groups metioned above received 284.1 mg/kg daily aluminum chloride(AlCl3)hexahydrate for the first four weeks;Then for the last eight weeks,they were given 1.0 ml/d saline,and flavonoids at related doses respectively.The organ coefficients,the content of malondialdehyde(MDA),the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and ATPase in liver and kidney of rats were determined in the end of experiment.Results Aluminum intake caused significant increase of MDAand decrease of the activities of SOD,GSH-Px and ATPase compared with control group(P<0.05)in liver and kidney of rats.In liver,there were significantly higher SOD activity in the 200 mg/kg rutin group and higher GSH-Px activity in the 200 mg/kg rutin group,200 mg/kg puerarin group,silymarin groups compared with those in the AlCl3group(P<0.05);There was no significantdifference in MDA content between the AlCl3group and the flavonoids intervention groups.In kidney,MDA content was significantly lower in the flavonoids intervention groups,while SOD and GSH-Px activity were significantly higher in the 200mg/kg rutin group compared with those in the AlCl3group(P<0.05).In liver,the activity of Na+K+-ATPase in the 200 mg/kg rutin group and 200 mg/kg silymarin group,Ca2+-ATPase in all 200 mg/kg flavonoids intervention groups,Mg2+-ATPase in the200 mg/kg puerarin group,200 mg/kg silymarin group and rutin groups were significantly higher compared with those in the AlCl3group(P<0.05).In kidney,the activity of Na+K+-ATPase in the 200 mg/kg rutin group and 200 mg/kg silymarin group,Ca2+-ATPase in the 200 mg/kg puerarin group and 200mg/kg silymarin group,Mg2+-ATPase in the 200 mg/kg rutin group and silymarin groups were significantly higher compared with those in the AlCl3group(P<0.05).With the increase of exposure doses of rutin,puerarin and silymarin,the MDA content showed a downward trend,while the levels of SOD,GSH-Px activities and Na+K+-ATPase,Mg2+-ATPase,Ca2+-ATPase showed an upward trend in liver and kidney of the intervention group.Conclusion Within certain dosage,flavonoids(rutin,puerarin,silymarin)can protect the antioxidant system in liver and kidney of rats exposed to aluminum,thus lessen the injury of liver and kidney effectively.

【基金】 山东省自然科学基金(ZR2017MH034)
  • 【文献出处】 环境与健康杂志 ,Journal of Environment and Health , 编辑部邮箱 ,2018年10期
  • 【分类号】R114
  • 【下载频次】35
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