Determination of nickel in urine by three-magnetic field adjustable Zeeman effect-graphite furnace atomic absorption spectrometry
【摘要】 目的建立尿中镍的三磁场可调塞曼效应-石墨炉原子吸收光谱法。方法利用三磁场可调塞曼效应石墨炉原子吸收光谱法,用不含镍的尿液来配制工作曲线,以磷酸二氢铵和硝酸镁为混合基体改进剂来测定尿中镍,可以直接计算尿中镍的浓度。结果在4～20μg/L的线性范围内,所得尿中镍的回归方程为y=0.006 33x+0.020 16,r=0.998 2。该方法的检出限为0.1μg/L,回收率在99.0%～100.1%之间,RSD为1.11%～1.45%。结论该方法灵敏度高,精密度好且线性范围宽,可以完成样品浓度变化范围很大的校正测定,不会出现塞曼反转现象,吸收信号无双峰,结果准确、可靠,适用于基层单位对尿中镍浓度的测定。
【Abstract】 Objective To establish a method for determination of nickel in urine by three-field adjustable Zeeman effect-graphite furnace atomic absorption spectrometry. Methods The three-magnetic field adjustable Zeeman effect-graphite furnace atomic absorption spectrometry was used to determine nickel in urine samples by using ammonium hydrogen phosphate and magnesium nitrate as the mixed matrix modifier. The working curve was prepared by urine solution without nickel,and the concentration of nickel in urine could be calculated directly. Results The linear range of nickel was 4-20 μg/L,the regression equation of nickel in urine was y=0.006 33 x+0.020 16,r=0.998 2. The limit of detection was 0.1 μg/L. The rates of recovery were between 99.0% and 100.1%,the RSDs of this method were 1.11%-1.45%. Conclusion This method has high sensitivity,good precision and wide linear range,it can complete the correction and determination with a wide range of sample concentration,without phenomenon of Zeeman inversion,and the absorption signal is not bimodal,and it is suitable for the determination of nickel in urine for basic units.
- 【文献出处】 环境与健康杂志 ,Journal of Environment and Health , 编辑部邮箱 ,2018年10期