Study on Modification of Collagen by Fluorescent Hyperbranched Polymer(HMEAP)
【摘要】 新型非传统荧光材料是目前研究的热点,本文制备了系列不同分子量的非传统荧光超支化聚合物(HMEAP),采用核磁共振、荧光分析、凝胶渗透色谱等表征手段对其进行结构及性能的研究。并将其应用于山羊酸皮鞣制,以鞣制后收缩温度的增加及皮革增厚率为考察指标,优化出了荧光超支化聚合物(HMEAP)对皮胶原蛋白的改性条件。实验结果表明,当HMEAP重均分子量约为17000,其鞣制效果最佳,与胶原蛋白作用条件为pH为5～6,聚合物用量约为6%,最佳反应温度为25～35℃,反应时间为4 h。HMEAP鞣制后皮革收缩温度可提高22.1℃,增厚率可达到35.6%,改性后胶原蛋白在生物医药方面应用具有广阔的应用前景。
【Abstract】 Recently, a new kind of unconventional fluorescent material has been a hot topic. The unconventional fluorescent hyperbranched polymers(HMEAP) were synthesized successfully, and were characterized by ~1H NMR, fluorescence spectral measurement and GPC. The optimum modification conditions were obtained by measuring the shrinkage temperature and thickness rate of leather after tannage, after the hyperbranched polymers being added into tanned pickling goat skin. The results showed that HMEAP had the best tanning effect when the average molecular weight of HMEAP was about 17000. The optimum conditions of interaction with collagen were as follows: pH was 5～6, the amount of polymer was about 6%, the reaction temperature was 25～35℃ and the reaction time was 4 h.After HMEAP tanning, the shrinkage temperature was increased by 22.1℃, and the thickness rate of leather reached to 35.6%. The modified collagen has broad application prospects in area of biomedicine.
- 【文献出处】 皮革与化工 ,Leather and Chemicals , 编辑部邮箱 ,2021年04期